3LFL
Crystal Structure of human Glutathione Transferase Omega 1, delta 155
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54060 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.009, 201.942, 53.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.505 - 2.100 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.128 | 0.370 |
| Number of reflections | 44498 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 94.4 | 80.7 |
| Redundancy | 8.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 0.2M trimethyl amine N-oxide dihydrate, 20% (w/v) polyethylene glycol monomethyl ether 2000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
