3LFK
A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-30 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.130, 88.335, 115.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.250 - 1.600 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.22800 |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.041 | 0.497 |
Number of reflections | 89296 | |
<I/σ(I)> | 45.8 | 4.4 |
Completeness [%] | 88.2 | 100 |
Redundancy | 8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 22-26% PEG 550MME, 80 mM ammonium sulfate, 85 mM citric acid, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |