3LEX
2F5 Epitope scaffold elicited anti-HIV-1 monoclonal antibody 11F10 in complex with HIV-1 GP41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.543, 70.962, 78.265 |
| Unit cell angles | 90.08, 90.02, 90.29 |
Refinement procedure
| Resolution | 25.900 - 1.970 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ley |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 2.040 |
| High resolution limit [Å] | 1.960 | 1.970 |
| Number of reflections | 49925 | |
| <I/σ(I)> | 33 | 12.9 |
| Completeness [%] | 94.6 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 4000, 0.08 M CH3COONA, 0.04 M, TRIS PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
