3LEW

Crystal structure of SusD-like carbohydrate binding protein (YP_001298396.1) from Bacteroides vulgatus ATCC 8482 at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97954
Spacegroup name	C 1 2 1
Unit cell lengths	191.772, 46.707, 73.524
Unit cell angles	90.00, 106.55, 90.00

Refinement procedure

Resolution	39.368 - 1.700
R-factor	0.146
Rwork	0.145
R-free	0.17000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.368		1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.070	0.037	0.627
Number of reflections	68815	7051	6661
<I/σ(I)>	11.49	28.4	2.1
Completeness [%]	99.5	98.5	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	5.0000% polyethylene glycol 3000, 32.0000% polyethylene glycol 400, 0.1M MES pH 6.5, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K