3LER
Crystal Structure of Dihydrodipicolinate Synthase from Campylobacter jejuni subsp. jejuni NCTC 11168
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.185, 85.304, 199.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.207 - 1.840 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 107674 | |
| <I/σ(I)> | 9.1 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M NaCl, 0.1 M Na/K phosphate pH 6.2, 20% (w/v) PEG-1000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
