3LEF
Crystal structure of HIV epitope-scaffold 4E10_S0_1Z6NA_001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 107 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 56.410, 56.410, 324.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.780 - 2.300 |
| R-factor | 0.25978 |
| Rwork | 0.258 |
| R-free | 0.29042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Computationally-derived model of the epitope-scaffold based on 1Z6N |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.373 |
| Number of reflections | 13770 | |
| <I/σ(I)> | 9 | 3.9 |
| Completeness [%] | 93.7 | 91.5 |
| Redundancy | 8.4 | 6.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Na/K tartrate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
