3LED
Crystal structure of 3-oxoacyl-(acyl carrier protein) synthase III from Rhodopseudomonas palustris CGA009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.997, 114.790, 60.429 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.450 |
| R-factor | 0.14593 |
| Rwork | 0.145 |
| R-free | 0.16843 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.064 | 0.343 |
| Number of reflections | 133399 | |
| <I/σ(I)> | 40.4 | 4.62 |
| Completeness [%] | 99.8 | 97 |
| Redundancy | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Sodium Formate, 20%PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
