3LDU
The crystal structure of a possible methylase from Clostridium difficile 630.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.059, 74.047, 64.061 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 23.795 - 1.700 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.21050 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.807 |
| Number of reflections | 41197 | |
| <I/σ(I)> | 35.9 | 1.88 |
| Completeness [%] | 98.4 | 98.1 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M MES, 15% PEG 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
