3LDT
Crystal structure of an Outer membrane protein(OmpA)from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 78.062, 78.062, 140.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.070 - 2.300 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 10001 | |
| <I/σ(I)> | 19.9 | 16.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.5 | 29.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 292 | 15% Isopropanol, 1.3M Ammonium Sulpahte , pH 7.0, EVAPORATION, temperature 292K |
