3LDO
Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9730 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.615, 74.563, 89.952 |
| Unit cell angles | 90.00, 98.54, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.950 |
| R-factor | 0.20563 |
| Rwork | 0.202 |
| R-free | 0.25558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.316 |
| Number of reflections | 55779 | |
| <I/σ(I)> | 15.1 | 3.5 |
| Completeness [%] | 98.3 | 89.8 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris buffer, 25% Peg3350, 0.1M Na,K tartrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
