3LD7
Crystal structure of the Lin0431 protein from Listeria innocua, Northeast Structural Genomics Consortium Target LkR112
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97814 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.360, 54.782, 71.733 |
| Unit cell angles | 90.00, 111.60, 90.00 |
Refinement procedure
| Resolution | 31.547 - 1.547 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.399 |
| Number of reflections | 84087 | |
| <I/σ(I)> | 34.3 | 3.4 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under paraffin oil | 8.2 | 291 | 0.072M sodium phosphate monobasic, 1.728M potassium phosphate dibasic, pH 8.2, microbatch under paraffin oil, temperature 291K |
