3LCZ
B.licheniformis Anti-TRAP can assemble into two types of dodecameric particles with the same symmetry but inverted orientation of trimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.28 |
| Spacegroup name | H 3 |
| Unit cell lengths | 108.076, 108.076, 49.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.760 - 2.060 |
| R-factor | 0.21661 |
| Rwork | 0.213 |
| R-free | 0.28578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bx9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.790 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.062 | 0.355 |
| Number of reflections | 13005 | |
| <I/σ(I)> | 19.2 | 4.1 |
| Completeness [%] | 97.8 | 95.1 |
| Redundancy | 5.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M Bis-Tris buffer pH 5.5-6.0 and 23-27 % of poly(ethylene) glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
