3LCY
Titin Ig tandem domains A164-A165
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8088 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.086, 103.821, 116.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.590 - 2.500 |
| R-factor | 0.2035 |
| Rwork | 0.203 |
| R-free | 0.23980 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX (dev_276) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.060 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.111 | 0.536 |
| Number of reflections | 32969 | |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 99.4 | 96 |
| Redundancy | 11.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes PH 7.5, 10% iso-propanol 8.5% PEG20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
