3LCN
Nab2:Gfd1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.833, 121.762, 37.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.073 - 2.000 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v75 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.803 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.335 |
| Number of reflections | 17741 | |
| <I/σ(I)> | 13.5 | 4.9 |
| Completeness [%] | 98.9 | 95.3 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 150 mM Zn acetate, 12% PEG400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
