3LC8
Crystal structure of the cytoplasmic tail of (pro)renin receptor as a MBP fusion (Maltose-free form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.780, 112.700, 175.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.745 - 2.000 |
| R-factor | 0.19732 |
| Rwork | 0.195 |
| R-free | 0.25741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jw4 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.745 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.529 |
| Number of reflections | 63627 | |
| <I/σ(I)> | 15.15 | 4 |
| Completeness [%] | 97.8 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 20% PEG 4000, 0.2M Magnesium Chloride, 0.1M Tris, pH 8.5, EVAPORATION, temperature 293K |
