3LBY
Crystal structure of SMU.1697c, a putative methyltransferase from streptococcus mutans in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.210, 92.210, 107.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.601 - 2.000 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.24410 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 35822 | |
| Completeness [%] | 96.5 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Bis-tris pH5.5, 1.0M Na Formate, 15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
