3LBD
LIGAND-BINDING DOMAIN OF THE HUMAN RETINOIC ACID RECEPTOR GAMMA BOUND TO 9-CIS RETINOIC ACID
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.666, 60.666, 155.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.400 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.26700 |
| Structure solution method | MR |
| Starting model (for MR) | 2lbd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.000 * |
| Data reduction software | MARXDS |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 * | 0.300 * |
| Number of reflections | 11390 | |
| <I/σ(I)> | 25.43 | 7.29 |
| Completeness [%] | 98.5 | 97.8 |
| Redundancy | 4.45 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 17 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.0 (mg/ml) | |
| 10 | 1 | reservoir | PIPES | 0.1 (M) | |
| 11 | 1 | reservoir | 0.9-1.1 (M) | ||
| 2 | 1 | drop | 9-cis retinoic acid | 0.4 (mM) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | drop | dithiothreitol | 15 (mM) | |
| 5 | 1 | drop | 500 (mM) | ||
| 6 | 1 | drop | n-dodecyl-beta-D-maltoside | 0.15 (mM) | |
| 7 | 1 | drop | CHAPS | 2 (mM) | |
| 8 | 1 | drop | glycerol | 4 (%) | |
| 9 | 1 | drop | ethanol | 2 (%) |
