3LB9
Crystal structure of the B. circulans cpA123 circular permutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-20 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.810, 114.970, 65.030 |
| Unit cell angles | 90.00, 113.47, 90.00 |
Refinement procedure
| Resolution | 19.880 - 3.000 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hv1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.688 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.800 |
| Rmerge | 0.147 | 0.412 |
| Number of reflections | 10649 | |
| <I/σ(I)> | 10.2 | 2.6 |
| Completeness [%] | 92.0 | 99.8 |
| Redundancy | 1147 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 13-20 % (NH4)2SO4 40 mM Tris-HCl, pH 8, VAPOR DIFFUSION, temperature 298K |
