3LAZ
The crystal structure of the N-terminal domain of D-galactarate dehydratase from Escherichia coli CFT073
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 106.279, 106.279, 51.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.529 - 1.921 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24510 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.070 | 0.743 |
| Number of reflections | 22935 | |
| <I/σ(I)> | 52.5 | 1.6 |
| Completeness [%] | 99.6 | 94.8 |
| Redundancy | 13.4 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris pH 3.0M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
