3LAT
Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.453, 99.453, 148.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.770 - 1.700 |
| R-factor | 0.14416 |
| Rwork | 0.143 |
| R-free | 0.16518 |
| Structure solution method | SIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.071 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.330 |
| Number of reflections | 82089 | |
| <I/σ(I)> | 19.6 | 2.4 |
| Completeness [%] | 99.4 | |
| Redundancy | 10 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 22% butanediol, 0.1M imidazole, 0.15M zinc acetate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
