3LAE
The crystal structure of a functionally unknown conserved protein from Haemophilus influenzae Rd KW20
Replaces: 2O1RExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 25.604, 77.563, 79.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.692 - 1.453 |
| R-factor | 0.1667 |
| Rwork | 0.164 |
| R-free | 0.22220 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.062 | 0.622 |
| Number of reflections | 14271 | |
| <I/σ(I)> | 38.7 | 1.9 |
| Completeness [%] | 99.4 | 94.3 |
| Redundancy | 7.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.4M NaH2PO4, 1.6M K2HPO4, 0.1M Imidazole, 0.2M NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
