3LAA
Crystal structure of the trimeric autotransporter adhesin head domain BpaA from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 50.718, 50.718, 136.496 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 1.350 |
| R-factor | 0.115 |
| Rwork | 0.114 |
| R-free | 0.12800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3la9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.075 | 0.399 |
| Number of reflections | 28462 | |
| <I/σ(I)> | 16.5 | 2.14 |
| Completeness [%] | 98.9 | 81.7 |
| Redundancy | 4.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | JCSG+ C12, 10% PEG 1000, 10% PEG 8000, crystal tracking ID 203139c12, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
