3LA5
X-ray crystal structure of mc6 RNA Riboswitch bound to azacytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.845, 125.105, 24.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.080 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y26 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.229 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.083 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.274 |
| Number of reflections | 23579 | |
| <I/σ(I)> | 16.88 | 5.25 |
| Completeness [%] | 94.4 | 80.7 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 3M 1,6-hexanediol, 0.1M Tris-HCl (pH 9.0), 200mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
