3L9Q
Crystal structure of human polymerase alpha-primase p58 iron-sulfur cluster domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2009-02-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.441, 53.050, 88.844 |
| Unit cell angles | 90.00, 115.09, 90.00 |
Refinement procedure
| Resolution | 37.321 - 1.698 |
| R-factor | 0.1317 |
| Rwork | 0.130 |
| R-free | 0.16900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (2009_02_15_2320_3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 49683 | |
| <I/σ(I)> | 21.2 | 6.9 |
| Completeness [%] | 97.5 | 84.9 |
| Redundancy | 7.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1 M Tris pH 8.5, 15% PEG 3250, 0.2 M LiSO4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
