3L9K
Insights into dynein assembly from a dynein intermediate chain-light chain roadblock structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 133.15 |
| Detector technology | CCD |
| Collection date | 2009-08-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 |
| Unit cell lengths | 107.180, 107.180, 65.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.800 - 3.000 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hz5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.909 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.820 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.442 |
| Number of reflections | 16845 | |
| <I/σ(I)> | 7.1 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277.15 | 1 M sodium citrate, 100 mM sodium chloride, 100 mM Tris., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
