3L9G
Urate oxidase complexed with uric acid and chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.948 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.737, 94.941, 104.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.750 |
| R-factor | 0.1794 |
| Rwork | 0.175 |
| R-free | 0.20800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l8w |
| RMSD bond length | 0.049 |
| RMSD bond angle | 0.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.200 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 | 0.201 |
| Number of reflections | 39071 | |
| <I/σ(I)> | 33.1 | 4.1 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 8 | 291 | 0.05M TRIS BUFFER PH 8, 8% PEG 8000, BATCH, temperature 291K |
