3L93
Phosphopantetheine adenylyltransferase from Yersinia pestis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.511, 115.511, 117.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.300 - 2.160 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l92 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.667 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 2.210 |
| High resolution limit [Å] | 2.160 | 2.170 |
| Rmerge | 0.065 | 0.764 |
| Number of reflections | 16233 | |
| <I/σ(I)> | 15.7 | 2.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 60% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
