3L8Z
H-Ras wildtype new crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.28350 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.082, 88.082, 133.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 1.440 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.911 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.036 | 0.335 |
| Number of reflections | 34928 | |
| <I/σ(I)> | 24.9 | 3.59 |
| Completeness [%] | 96.4 | 82.1 |
| Redundancy | 10.69 | 4.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 17% PEG6000, 200mM CaCl2, 30mM TRIS/HCL, 5mM MgCl2, 2mM DTE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
