3L8W
Urate oxidase from aspergillus flavus complexed with xanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.527, 94.980, 104.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.000 |
| R-factor | 0.124 |
| Rwork | 0.121 |
| R-free | 0.14400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.083 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.160 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.053 | 0.201 |
| Number of reflections | 153725 | |
| <I/σ(I)> | 18.3 | 3.7 |
| Completeness [%] | 95.1 | 99 |
| Redundancy | 5.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 8 | 291 | Tris buffer 0.05M, 8% PEG 8000, pH 8.0, Batch, temperature 291K |
