3L87
The Crystal Structure of smu.143c from Streptococcus mutans UA159
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-30 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.589, 55.621, 85.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.670 - 2.000 |
| R-factor | 0.21863 |
| Rwork | 0.215 |
| R-free | 0.28524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2os3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.769 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 2.270 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 10849 | |
| Completeness [%] | 68.0 | 30.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 20% PEG 3350 , 0.1M HEPES pH7.5, 0.1M Lithium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
