3L84
High resolution crystal structure of transketolase from Campylobacter jejuni subsp. jejuni NCTC 11168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.493, 71.181, 69.416 |
| Unit cell angles | 90.00, 109.77, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.360 |
| R-factor | 0.13268 |
| Rwork | 0.132 |
| R-free | 0.15171 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.070 | 0.690 |
| Number of reflections | 127117 | |
| <I/σ(I)> | 35.8 | 2.3 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | Tacsimate, Hepes, 10 % 5000 PEGMMe, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
