3L7Y
The Crystal Structure of SMU.1108c from Streptococcus mutans UA159
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-13 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.094, 80.198, 47.937 |
| Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
| Resolution | 55.177 - 1.996 |
| R-factor | 0.1905 |
| Rwork | 0.184 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rlm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.379 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.200 | 2.150 |
| High resolution limit [Å] | 1.996 | 1.996 |
| Number of reflections | 17580 | |
| Completeness [%] | 90.2 | 55.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M tris pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
