3L7P
Crystal structure of SMU.1657c, Putative nitrogen regulatory protein PII from streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-03 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.51 |
| Spacegroup name | P 41 |
| Unit cell lengths | 60.805, 60.805, 147.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.900 - 2.000 |
| R-factor | 0.26147 |
| Rwork | 0.258 |
| R-free | 0.32661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.073 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 35955 | |
| Completeness [%] | 99.8 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15% PEG 3350, 0.1 MES pH6.5, 0.83M NaNO3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
