3L7M
Structure of the Wall Teichoic Acid Polymerase TagF, H548A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 223.510, 223.510, 101.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.943 - 2.850 |
| R-factor | 0.1941 |
| Rwork | 0.191 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l7i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.168 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.064 | 0.582 |
| Number of reflections | 482401 | |
| <I/σ(I)> | 25.9 | 1.4 |
| Completeness [%] | 89.8 | 59.7 |
| Redundancy | 8.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 1.75M ammonium phosphate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
