3L6V

Crystal Structure of the Xanthomonas campestris Gyrase A C-terminal Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-16
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.97960, 0.96430
Spacegroup name	P 1
Unit cell lengths	52.494, 58.968, 74.415
Unit cell angles	79.00, 79.78, 69.62

Refinement procedure

Resolution	29.000 - 2.190
R-factor	0.22123
Rwork	0.219
R-free	0.27059
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.250
High resolution limit [Å]	2.190	2.190
Rmerge	0.044	0.109
Number of reflections	40325
Completeness [%]	96.5	92.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	200mM ammonium sulfate, 100mM Bis-Tris pH 5.2-5.9, 25-29% (v/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	200mM ammonium sulfate, 100mM Bis-Tris pH 5.2-5.9, 25-29% (v/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K