3L6U

Crystal structure of abc-type sugar transport system, Periplasmic component from exiguobacterium sibiricum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	66.932, 57.309, 73.131
Unit cell angles	90.00, 106.70, 90.00

Refinement procedure

Resolution	24.850 - 1.900
R-factor	0.235
Rwork	0.235
R-free	0.26900
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.900
Rmerge	0.063
Number of reflections	41731
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% PEG 3350, 0.1 Bis-Tris, 0.1M Lithium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K