3L6Q

Crystal structure of the N-terminal domain of HSP70 from Cryptosporidium parvum (CGD2_20)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97899
Spacegroup name	P 21 21 21
Unit cell lengths	71.089, 83.078, 131.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 2.290
R-factor	0.193
Rwork	0.191
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kvg
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.101	0.052	0.744
Number of reflections	41916
<I/σ(I)>	7.2
Completeness [%]	99.9	99	100
Redundancy	7.4	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	30% PEG 400 0.2M NH4SO4 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K