3L6P
Crystal Structure of Dengue Virus 1 NS2B/NS3 protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9497 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.721, 60.848, 161.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.950 - 2.200 |
| R-factor | 0.242 |
| Rwork | 0.239 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fom |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 14424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.1M HEPES pH 7.5, 12% PEG 3350 and 0.005M each of NiCl2, CdCl2, MgCl2 and CoCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
