3L6I

Crystal structure of the uncharacterized lipoprotein yceb from e. coli at the resolution 2.0a. northeast structural genomics consortium target er542

Replaces:  3EYR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	C 2 2 2
Unit cell lengths	109.050, 174.550, 43.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.793 - 2.011
R-factor	0.2072
Rwork	0.205
R-free	0.24670
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.251
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.890	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.054	0.444
Number of reflections	50815
<I/σ(I)>	39.6	6
Completeness [%]	99.8	100
Redundancy	7.6	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	2.3M NA MALONATE, 0.05M BIS TRIS, PROPANE PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K