3L6G
Crystal structure of lactococcal OpuAC in its open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.131, 68.131, 109.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.073 - 1.900 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b4l |
RMSD bond length | 0.017 |
RMSD bond angle | 1.428 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.2.0) |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.010 | |
High resolution limit [Å] | 1.900 | 5.660 | 1.900 |
Rmerge | 0.099 | 0.034 | 0.542 |
Number of reflections | 20918 | 884 | 3278 |
<I/σ(I)> | 15.62 | 37.2 | 3.9 |
Completeness [%] | 99.7 | 98.1 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M sodium iodide, 0.1 M Bis-Tris propane pH 8.5, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |