3L6A

Crystal structure of the C-terminal region of Human p97

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-02
Detector	MAR CCD 130 mm
Wavelength(s)	1.54180
Spacegroup name	P 21 21 21
Unit cell lengths	31.084, 102.270, 112.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.450 - 2.000
R-factor	0.203
Rwork	0.200
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.196
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.12)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.078
Number of reflections	28463
<I/σ(I)>	13.5
Completeness [%]	99.9
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	2.1M (NH4)2SO4, 0.1M MES pH 6.0, 2% PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	2.1M (NH4)2SO4, 0.1M MES pH 6.0, 2% PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 289K