3L63
Crystal structure of camphor-bound P450cam at low [K+]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.362, 98.194, 54.197 |
| Unit cell angles | 90.00, 103.86, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.16589 |
| Rwork | 0.164 |
| R-free | 0.19652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cp4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.790 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 0.529 |
| Number of reflections | 57384 | |
| <I/σ(I)> | 12.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 279 | 12-22% PEG 8000, 0.05M Tris, 4-8mM camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
