3L5O

Crystal structure of protein with unknown function from DUF364 family (ZP_00559375.1) from Desulfitobacterium hafniense DCB-2 at 2.01 A resolution

Replaces:  2H1Q

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-29
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 61
Unit cell lengths	120.640, 120.640, 75.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.977 - 2.010
R-factor	0.173
Rwork	0.171
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.428
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.977		2.080
High resolution limit [Å]	2.010	4.320	2.010
Rmerge	0.059	0.039	0.668
Rmeas	0.066	0.042	0.815
Total number of observations	311344
Number of reflections	41624	8192	7832
<I/σ(I)>	12.26	36.3	1.8
Completeness [%]	99.7	99.2	98.1
Redundancy	7.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	1.0M LiCl, 0.1M citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K