3L5N
Staphylococcal Complement Inhibitor (SCIN) in complex with Human Complement Component C3b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 128.026, 128.026, 468.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.622 - 7.536 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Entries 2IO7 2qff |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 6.210 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.099 | 0.522 |
| Number of reflections | 4682 | |
| <I/σ(I)> | 8.5 | 1.75 |
| Completeness [%] | 69.5 | 29.2 |
| Redundancy | 7.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein solution 5mg/ml, 0.1M HEPES-NaOH, 30%(v/v) Jeffamine ED-2001-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
