3L57
Crystal Structure of the Plasmid pCU1 TraI Relaxase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.280, 58.520, 87.120 |
| Unit cell angles | 90.00, 83.88, 90.00 |
Refinement procedure
| Resolution | 48.492 - 2.293 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omh Plasmid R388 TrwC Relaxase Domain |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.724 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.999 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 22081 | 3362 |
| <I/σ(I)> | 18.25 | 4.5 |
| Completeness [%] | 96.6 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 250mM triNa citrate, 22% PEG 3350, 5mM MgCl2, vapor diffusion, hanging drop, temperature 293.15K |
