3L51
Crystal Structure of the Mouse Condensin Hinge Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-04-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.455, 96.741, 54.537 |
| Unit cell angles | 90.00, 92.84, 90.00 |
Refinement procedure
| Resolution | 29.133 - 1.506 |
| R-factor | 0.1459 |
| Rwork | 0.143 |
| R-free | 0.17290 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.133 | 1.600 |
| High resolution limit [Å] | 1.506 | 1.506 |
| Number of reflections | 53950 | 16418 |
| <I/σ(I)> | 21.43 | 11.68 |
| Completeness [%] | 96.5 | 90.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG 4000, 5% isopropanol, 20% glycerol, 100mM Tris-HCl pH 8.5, vapor diffusion, hanging drop, temperature 293K |
