3L4V

Crystal complex of N-terminal Human Maltase-Glucoamylase with kotalanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9175
Spacegroup name	P 21 21 21
Unit cell lengths	91.278, 109.693, 110.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.100
R-factor	0.18456
Rwork	0.182
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.583
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.140
High resolution limit [Å]	2.100	5.660	2.100
Rmerge	0.122	0.091	0.515
Number of reflections	65399
<I/σ(I)>	9.2
Completeness [%]	99.9	98.7	100
Redundancy	7.3	6.6	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20% PEG 3350, 0.2M sodium sulfate, pH 6.5, vapor diffusion, hanging drop, temperature 293K