3L4U
Crystal complex of N-terminal Human Maltase-Glucoamylase with de-O-sulfonated kotalanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9175 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.865, 109.866, 109.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.576 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.121 | 0.072 | 0.608 |
| Number of reflections | 84175 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 7 | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.2M sodium sulfate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
