3L4S
Crystal structure of C151G mutant of Glyceraldehyde 3-phosphate dehydrogenase 1 (GAPDH1) from methicillin resistant Staphylococcus aureus MRSA252 complexed with NAD and G3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.593, 102.936, 90.652 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 27.760 - 2.200 |
| R-factor | 0.19961 |
| Rwork | 0.197 |
| R-free | 0.24877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H48 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.699 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.550 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.400 |
| Number of reflections | 57048 | |
| <I/σ(I)> | 8.3 | 2.5 |
| Completeness [%] | 94.9 | 98.6 |
| Redundancy | 3.63 | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 (M) Tris-HCl, pH8.5, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
