3L4N
Crystal structure of yeast monothiol glutaredoxin Grx6
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.556, 57.132, 59.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.720 - 1.500 |
| R-factor | 0.18051 |
| Rwork | 0.179 |
| R-free | 0.20035 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.671 |
| Number of reflections | 27154 | |
| <I/σ(I)> | 35.13 | 2.26 |
| Completeness [%] | 96.7 | 94.1 |
| Redundancy | 9.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 19% polyethylene glycol monomethyl ether 5000, 0.1M 2-(N-morpholino)-ethane sulfonic acid (MES), pH 6.5, 10mM reduced glutathione (GSH), VAPOR DIFFUSION, HANGING DROP, temperature 289K |
